In this second updated edition, the editors have added critical reviews to a
collection of cutting-edge protocols for gene expression in bacteria, fungi,
plants, plant cells, animals, and animal cells. The review articles survey new
directions in recombinant gene expression research, technique, and application,
and point the way to using recombinant gene expression for metabolic engineering
and the production of non-protein molecules. The readily reproducible protocols
offer new host-vector systems, such as Anarctic bacteria and moderately
halophytic bacteria, vectors for chromosomal editing and metabolic engineering,
and information to aid in problem solving, strategy planning, and the promotion
of protein folding. Recombinant Gene Expression: Reviews and Protocols, Second
Edition offers