Hands-on researchers describe in step-by-step detail 73 proven laboratory
methods and bioinformatics tools essential for analysis of the proteome. These
cutting-edge techniques address such important tasks as sample preparation,
2D-PAGE, gel staining, mass spectrometry, and post-translational modification.
There are also readily reproducible methods for protein expression profiling,
identifying protein-protein interactions, and protein chip technology, as well
as a range of newly developed methodologies for determining the structure and
function of a protein. The bioinformatics tools include those for analyzing
2D-GEL patterns, protein modeling, and protein identification. All
laboratory-based protocols follow the successful Methods in Molecular Biology
series format, each offering step-by-step laboratory instructions, an
introduction outlining the principle behind the