Protein engineering is a fascinating mixture of molecular biology, protein
structure analysis, computation, and biochemistry, with the goal of developing
useful or valuable proteins. Divided into two sections, Protein Engineering
Protocols reviews rational protein design strategies and directed evolutionary
techniques, and their impact on protein engineering.
The first section presents design and computational strategies for protein
engineering focusing on designing a protein with desired properties, including
examples covering a wide range of engineering techniques, such as
protein-protein interactions, DNA binding, antibody mimics, and enzymatic
activity. The second section on evolutionary techniques provides step-by-step
instructions on library design and statistical assessment of library quality.
New methods for DNA shuffling as