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PCR Sequencing Protocols (Methods in Molecular Biology)
by Ralph Rapley (Author),
Humana Press (Publisher)
Product Specifications
Book Description
A superb, comprehensive collection of the most reliable protocols for PCR
sequencing-all developed and described by eminent researchers and scientists who
use them successfully every day. These protocols deal practically with those
problems accompanying the rapid reassociation of amplified complementary strands
by showing how sequencing reactions may be effectively modified, as well as
complex methods of primer manipulation that enable the affinity purification, or
its transcript production, of homogeneous single strands. They also provide all
the necessary details of PCR purification, primer labeling, the preparation of
sequencing gels, as well as of conventional methods for cloning PCR products
into vectors and their subsequent sequencing.
Book Specifications
ISBN-13 9780896033443
Language English
Binding Paper Back
Publisher Humana Press
Publishing Date 1996-08-19
Total Pages 221