John Walker and Ralph Rapley have collected a wide-ranging group of molecular
and biochemical techniques that are the most frequently used in medical and
clinical research, especially diagnostics. The authors-well-established
investigators who run their own research programs and use the methods on a
regular basis-outline the practical procedures for using them and describe a
variety of pertinent applications. Among the technologies presented are southern
and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid
chromatography, in situ hybridization, karyotyping, flow cytometry,
bioinformatics, genomics, and ribotyping. The applications include assays for
mutation detection, mRNA analysis, chromosome translocations, inborn errors of
metabolism, protein therapeutics, and gene therapy.