About the Book: G Protein Signaling Methods And Protocols
A collection of cutting-edge methods for investigating G protein signaling from
a variety of perspectives ranging from in vitro biochemistry to whole animal
studies. Among the readily reproducible techniques presented are those for the
purification of G proteins and effectors enzymes, assays of these purified G
proteins and effector enzymes, and for the study of G proteins interactions with
effectors in intact cells. Additional methods are provided for assaying G
protein coupled receptor structure, function, and localization; for studying the
physiological roles for endogenous G proteins; and for examining lipid and
phosphate modifications of RGS proteins