This detailed volume provides methods and techniques for detection after
blotting. Chapters guide readers through a number of variations on the theme of
protein transfer to solid support followed by detection, presenting adaptations
of traditional techniques, and original methods of protein blotting. Written for
the Methods in Molecular Biology series, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.Practical and authoritative, Detection of Blotted Proteins: Methods and
Protocols presents numerous techniques based on the Western blot, providing
detailed, readily reproducible methods, tips, and alternatives directly and
easily transferable to the laboratory