Great disparities exist between organisms with regard to the relative ease of
chromosomal mutagenesis and manipulation. In Chromosomal Mutagenesis, a team of
experts provide a variety of chromosomal manipulation techniques, including
insertional gene disruptions, gene knockouts, stimulated homologous
recombination techniques and other novel tools, for both prokaryotic and
eukaryotic organisms, and attempt to expand the genetic toolbox beyond model
organisms. Following the format of the highly successful Methods in Molecular
Biology format, each chapter offers step-by-step laboratory instructions, lists
of the necessary equipment and reagents, and tips on troubleshooting and
avoiding known pitfalls.
Comprehensive and cutting-edge, Chromosomal Mutagenesis covers state-of-the-art
techniques that are staged to expand, if not revolutionize,