Christine Schneider has assembled a unique collection of readily reproducible
protocols for the study of chaperonins, intracellular proteins critical to many
biological processes. Written by experienced investigators who have successfully
honed their methods to a fineness, the protocols focus on the purification of
chaperonins from different species along with their corresponding cofactors, and
on chaperonin activity assays for in vivo as well as in vitro work. Many
activity assays are given for GroEL, which can also be applied to mitochondrial
Hsp60. There are also assays for the eukaryotic chaperonin TRiC and handy
methods-for example, one for preparing labeled probes-that can be used for
various purposes and prove helpful