The use of thermodynamics in biological research can be equated to an energy
book-keeping system. While the structure and function of a molecule is
important, it is equally important to know what drives the energy force. These
methods look to answer: What are the sources of energy that drive the function?
Which of the pathways are of biological significance? As the base of
macromolecular structures continues to expand through powerful techniques of
molecular biology, such as X-ray crystal data and spectroscopy methods, the
importance of tested and reliable methods for answering these questions will
continue to expand as well. This volume presents sophisticated methods for
estimating the thermodynamic